CHARACTERISTICS OF MICROSPORES’ SPORADES IN TETRAPLOID MAIZE LINE KrP-1 

Lobanova Lyudmila Petrovna, Candidate of biological sciences, associate professor, sub-department of genetics, N. G. Chernyshevsky Saratov State University (83 Astrakhanskaya street, Saratov, Russia), E-mail: lobanova-lp@yandex.ru
Kolesova Alla Yur'evna, Candidate of biological sciences, head of department of genetics, “Botanical Garden” Educational and Research Center, N. G. Chernyshevsky Saratov State University (83 Astrakhanskaya street, Saratov, Russia),
E-mail: kolesovaau@yandex.ru
Shatskaya Ol'ga Aleksandrovna, Senior researcher, department of corn breeding and seed growing division, National Center of Grain named after P. P. Lukyanenko (Central Estate of KNIISH, Krasnodar, Russia), E-mail: o.shatskaya@mail.ru
Magerramov Shamil' Valekhovich, Postgraduate student, N. G. Chernyshevsky Saratov State University (83 Astrakhanskaya street, Saratov, Russia), E-mail: magerramov.1994@list.ru 

633.15: 576.354: 575. 224 

DOI

10.21685/2307-9150-2018-4-7 

Background. The study of the pollen development in the tetraploid maize line KrP-1 is important for assessing the possibility of its usage in breeding work. The work aimed on the study of the microspores’ sporades structure, the features of which largely determine the pollen quality and the fertilization success.
Materials and methods. The analysis of microspores tetrads in plants of the tetraploid maize line KrP-1 was carried out on temporary preparations stained with acetocarmine. The preparations were analyzed on an Axiostar-plus microscope at a magnification of 10×40. Photographing was performed at the same magnification using the Zoom browser visualization program.
Results. Data were obtained on the quantity and quality of the resulting meiosis products at the tetrad stage. Tetrads of microspores with different types of cell partitioning and sporades with a number of cells different from four were found. The formation of abnormal structures in tetradogenesis (monades, diades, triades, tetrades with morphological abnormalities and polyades) may cause partial sterility of pollen or lead to the formation of unreduced and aneuploid gametes. The possibility of the formation of microspores of anomalously elongated shape in sporades with different numbers of cells was revealed. The latter implies the presence of a specific mutation that changes the shape of meiotic cells in the studied maize line.
Conclusions. Sporades morphology in tetraploid plants are extremely diversified and are due to abnormalities in nuclear fission and cell walls initiation in meiosis. However, a high percentage of formation of typical tetrads of microspores indicates the possibility of the formation of a sufficiently large amount of fertile pollen in this line and its usage in breeding. 

Key words

tetraploid corn, microsporogenesis, abnormal Sporades, unreduced and aneuploid microspores, atypical form of microspores 

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